000			03345nam a22004935i 4500
001			978-3-319-00807-3
003			DE-He213
005			20140220082839.0
007			cr nn 008mamaa
008			130808s2013 gw | s |||| 0|eng d
020			_a9783319008073 _9978-3-319-00807-3
024	7		_a10.1007/978-3-319-00807-3 _2doi
050		4	_aQD241-441
072		7	_aPSB _2bicssc
072		7	_aSCI013040 _2bisacsh
072		7	_aSCI007000 _2bisacsh
082	0	4	_a547 _223
100	1		_aSchirwitz, Christopher. _eauthor.
245	1	0	_aPurification of Peptides in High-Complexity Arrays _h[electronic resource] : _bA New Method for the Specific Surface Exchange and Purification of Entire Peptide Libraries / _cby Christopher Schirwitz.
264		1	_aCham : _bSpringer International Publishing : _bImprint: Springer, _c2013.
300			_aXIII, 132 p. 59 illus., 53 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aSpringer Theses, Recognizing Outstanding Ph.D. Research, _x2190-5053
505	0		_aIntroduction -- Concept & State-of-the-Art -- Results & Discussion -- Conclusion -- Materials & Methods.
520			_aChristopher Schirwitz's thesis focuses on improving the quality of in situ synthesized high-complexity peptide micro arrays. Micro arrays containing proteins or small protein fragments in the form of peptides have become of great interest in proteomic research. With the help of these microarrays a large number of potential target molecules can be screened for interaction with a probe in a short timeframe. However, protein and peptide micro arrays are still lagging behind oligonucleotide arrays in terms of density, quality and manufacturing costs. A new approach developed at the German Cancer Research Center (DKFZ) has improved the synthesis of high-density peptide arrays. The current technology is capable of producing arrays with up to 40,000 different peptides per square cm by means of micro particle-based solid phase peptide synthesis. However, in situ synthesis approaches bear a conceptual disadvantage: The quality of the peptides is dependent on the efficiency of the synthesis so that peptide fragments are present in the resulting array among the desired full-length peptides. In peptide-protein interaction studies such peptide fragments. The central achievement of this thesis is the development of a new method allowing for the fast one-step purification of entire arrays without loss of resolution or spatial information. Christopher Schirwitz's work has resulted in a number of publications in high ranking journals.
650		0	_aChemistry.
650		0	_aBioorganic chemistry.
650		0	_aChemistry, Physical organic.
650		0	_aBiochemistry.
650	1	4	_aChemistry.
650	2	4	_aBioorganic Chemistry.
650	2	4	_aProtein Science.
650	2	4	_aPhysical Chemistry.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9783319008066
830		0	_aSpringer Theses, Recognizing Outstanding Ph.D. Research, _x2190-5053
856	4	0	_uhttp://dx.doi.org/10.1007/978-3-319-00807-3
912			_aZDB-2-CMS
999			_c96478 _d96478