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001 978-1-4614-3964-6
003 DE-He213
005 20140220082813.0
007 cr nn 008mamaa
008 120905s2013 xxu| s |||| 0|eng d
020 _a9781461439646
_9978-1-4614-3964-6
024 7 _a10.1007/978-1-4614-3964-6
_2doi
050 4 _aQH506
072 7 _aMBGR
_2bicssc
072 7 _aPSD
_2bicssc
072 7 _aSCI049000
_2bisacsh
072 7 _aMED067000
_2bisacsh
082 0 4 _a611.01816
_223
100 1 _aTeng, Quincy.
_eauthor.
245 1 0 _aStructural Biology
_h[electronic resource] :
_bPractical NMR Applications /
_cby Quincy Teng.
250 _a2nd ed. 2013.
264 1 _aBoston, MA :
_bSpringer US :
_bImprint: Springer,
_c2013.
300 _aXIV, 431 p. 152 illus., 30 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aBasic Principles of NMR -- Instrumentation -- NMR Sample Preparation -- Practical Aspects -- Multi-Dimensional Heteronuclear NMR Experiments -- Studies Of Small Biological Molecules -- Protein Structure Determination From NMR Data -- Protein Dynamics -- NMR-based Metabolomics.
520 _aOver the years since NMR was first applied to solve problems in structural biology, both NMR instrument hardware and methodology have been dramatically developed. This book offers a much-needed update to the 2005 first edition, and will be of critical importance to those who routinely use NMR to study various biological systems as well as a textbook. Thus, the book is organized with experimentalists in mind, whether they are instructors or students. For those who have minimal background in NMR structural biology, this book will provide fresh perspective and insight; those who are already well-versed in NMR research will find cutting edge updates and new information and methods that are useful to their research. Because understanding fundamental principles and concepts of NMR spectroscopy is essential for the application of NMR methods to research projects, the book begins with an introduction to basic NMR principles. Next, NMR instrumentation is discussed starting with hardware components. Topics include magnetic field homogeneity and stability, signal generation and detection, probe circuits, cryogenic probe, analog-to-digital conversion, and test equipment. A typical specification for a NMR spectrometer is also included in the chapter. There is also a chapter covering NMR sample preparation, a process that is often the bottleneck for the success of the NMR projects. Several routine strategies for preparing samples, especially for macromolecules as well as complexes are dealt with in detail.
650 0 _aMedicine.
650 0 _aBiotechnology.
650 0 _aBiochemistry.
650 1 4 _aBiomedicine.
650 2 4 _aMolecular Medicine.
650 2 4 _aBiomedicine general.
650 2 4 _aBiophysics and Biological Physics.
650 2 4 _aBiochemistry, general.
650 2 4 _aBiotechnology.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9781461439639
856 4 0 _uhttp://dx.doi.org/10.1007/978-1-4614-3964-6
912 _aZDB-2-SBL
999 _c95037
_d95037