000 03990nam a22005055i 4500
001 978-94-007-7545-9
003 DE-He213
005 20140220082532.0
007 cr nn 008mamaa
008 131212s2014 ne | s |||| 0|eng d
020 _a9789400775459
_9978-94-007-7545-9
024 7 _a10.1007/978-94-007-7545-9
_2doi
050 4 _aQC450-467
050 4 _aQC718.5.S6
072 7 _aPNFS
_2bicssc
072 7 _aPDND
_2bicssc
072 7 _aSCI078000
_2bisacsh
082 0 4 _a621.36
_223
100 1 _aMondal, Partha Pratim.
_eauthor.
245 1 0 _aFundamentals of Fluorescence Microscopy
_h[electronic resource] :
_bExploring Life with Light /
_cby Partha Pratim Mondal, Alberto Diaspro.
264 1 _aDordrecht :
_bSpringer Netherlands :
_bImprint: Springer,
_c2014.
300 _aXV, 218 p. 140 illus., 55 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aPreface -- Acknowledgements -- Dedication -- 1 Ray Optics, Wave Optics and Imaging System Designing -- 2 Basics of Electromagnetic Theory for Fluorescence Microscopy -- 3 Electric Field Effects in Optical Microscopy Systems -- 4 Quantum Description of Radiation Field and Optical Microscopy -- 5 Molecular Physics of Fluorescence Markers -- 6 Basics of Fluorescence and Photophysics -- 7 General Fluorescence Imaging Techniques -- 8 Multiphoton Fluorescence Microscopy -- 9 Super Resolution Fluorescence Microscopy -- 10 Image Reconstruction Methodologies for Fluorescence Microscopy -- 11 Future Prospective of Fluorescence Microscopy.- Appendix I -- Appendix II -- Appendix III.
520 _aThis book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence  factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications.  The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging.
650 0 _aPhysics.
650 0 _aSpectroscopy.
650 0 _aMicroscopy.
650 1 4 _aPhysics.
650 2 4 _aSpectroscopy and Microscopy.
650 2 4 _aBiological Microscopy.
650 2 4 _aBiophysics and Biological Physics.
650 2 4 _aOptics, Optoelectronics, Plasmonics and Optical Devices.
650 2 4 _aSpectroscopy/Spectrometry.
700 1 _aDiaspro, Alberto.
_eauthor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9789400775442
856 4 0 _uhttp://dx.doi.org/10.1007/978-94-007-7545-9
912 _aZDB-2-PHA
999 _c94061
_d94061