000 02834nam a22004815i 4500
001 978-3-319-02159-1
003 DE-He213
005 20140220082510.0
007 cr nn 008mamaa
008 131031s2014 gw | s |||| 0|eng d
020 _a9783319021591
_9978-3-319-02159-1
024 7 _a10.1007/978-3-319-02159-1
_2doi
050 4 _aQD71-142
072 7 _aPNF
_2bicssc
072 7 _aSCI013010
_2bisacsh
082 0 4 _a543
_223
100 1 _aSharp, Thomas Harry.
_eauthor.
245 1 0 _aBiomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo
_h[electronic resource] /
_cby Thomas Harry Sharp.
264 1 _aCham :
_bSpringer International Publishing :
_bImprint: Springer,
_c2014.
300 _aXVIII, 150 p. 85 illus., 55 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSpringer Theses, Recognizing Outstanding Ph.D. Research,
_x2190-5053
505 0 _aBiomolecules & Microscopy -- Materials and Methods -- Experimental Methods -- Imaging Methods -- Experimental Results -- Towards an Atomistic Structure of a De Novo Designed Peptide Fibre -- Developing Novel Probes for Correlative Light Electron Microscopy.
520 _aAs part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his PhD, Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.
650 0 _aChemistry.
650 0 _aAnalytical biochemistry.
650 0 _aMicroscopy.
650 0 _aBiomaterials.
650 1 4 _aChemistry.
650 2 4 _aAnalytical Chemistry.
650 2 4 _aBiological Microscopy.
650 2 4 _aBiomaterials.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783319021584
830 0 _aSpringer Theses, Recognizing Outstanding Ph.D. Research,
_x2190-5053
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-319-02159-1
912 _aZDB-2-CMS
999 _c92820
_d92820