000			03283nam a22005175i 4500
001			978-3-642-04609-4
003			DE-He213
005			20140220084527.0
007			cr nn 008mamaa
008			100301s2010 gw | s |||| 0|eng d
020			_a9783642046094 _9978-3-642-04609-4
024	7		_a10.1007/978-3-642-04609-4 _2doi
050		4	_aQR180-189.5
072		7	_aMJCM _2bicssc
072		7	_aMED044000 _2bisacsh
082	0	4	_a616.079 _223
100	1		_aBuchwalow, Igor B. _eauthor.
245	1	0	_aImmunohistochemistry: Basics and Methods _h[electronic resource] / _cby Igor B. Buchwalow, Werner Böcker.
264		1	_aBerlin, Heidelberg : _bSpringer Berlin Heidelberg, _c2010.
300			_aX, 153p. 39 illus. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
505	0		_aAntibodies for Immunohistochemistry -- Antibody Labeling and the Choice of the Label -- Probes Processing in Immunohistochemistry -- Working with Antibodies -- Background Staining, Autofluorescence and Blocking Steps -- Immunostaining Enhancement -- Multiple Multicolor Immunoenzyme Staining -- Multiple Immunofluorescence Staining -- Antigen Detection on Tissues Using Primary Antibody Raised in the Same Species -- Probes for Staining Specific Cellular Organelles -- The Use of Epitope Tags in Histochemistry -- Immunohistochemistry at the Ultrastructural Level -- Diagnostic Immunohistochemistry -- A Picture Is Worth a Thousand Words.
520			_aWhile many excellent texts and monographs exist which cover various aspects of immunohistochemistry, the lack of a concise comprehensive guide to using the field’s methods was a major motivation for the authors to write this book. The authors carefully selected the methods and easy-to-adopt protocols discussed. In addition to estabished techniques, special attention was paid to current developments in immunohistochemistry such as antigen retrieval, signal amplification, the use of epitope tags, multiple immunolabeling, and diagnostic immunohistochemistry. All procedures examined have been tested by the authors; many are routinely used in daily practice in their institute. Each chapter starts with a short introduction to the respective method’s underlying principles before presenting step-by-step protocols and instructions, including precise recipes for all materials needed. Thus, the book is suitable for a broad audience, ranging from students and technical assistants to experienced researchers.
650		0	_aMedicine.
650		0	_aImmunology.
650		0	_aMonoclonal antibodies.
650		0	_aMedical laboratories.
650		0	_aCytology.
650		0	_aCytology _xResearch_xMethodology.
650	1	4	_aBiomedicine.
650	2	4	_aImmunology.
650	2	4	_aBiological Techniques.
650	2	4	_aCell Biology.
650	2	4	_aLaboratory Medicine.
650	2	4	_aAntibodies.
700	1		_aBöcker, Werner. _eauthor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9783642046087
856	4	0	_uhttp://dx.doi.org/10.1007/978-3-642-04609-4
912			_aZDB-2-SBL
999			_c111616 _d111616