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001 978-94-007-0828-0
003 DE-He213
005 20140220083831.0
007 cr nn 008mamaa
008 110614s2011 ne | s |||| 0|eng d
020 _a9789400708280
_9978-94-007-0828-0
024 7 _a10.1007/978-94-007-0828-0
_2doi
050 4 _aQD71-142
072 7 _aPNF
_2bicssc
072 7 _aSCI013010
_2bisacsh
082 0 4 _a543
_223
100 1 _aIvanov, Alexander R.
_eeditor.
245 1 0 _aSample Preparation in Biological Mass Spectrometry
_h[electronic resource] /
_cedited by Alexander R. Ivanov, Alexander V. Lazarev.
264 1 _aDordrecht :
_bSpringer Netherlands,
_c2011.
300 _aXXIX, 1089p. 253 illus., 104 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aIntroduction: Sample Preparation – Boring but Important! Section I. Traditional and improved techniques in sample preparation for proteomics -- Section II. Methods for Improved Proteolytic Digestion -- Section III. Methods for Tissue, Cell, and Organelle Preparation and Analysis -- Section IV. 2D Gel-based Proteomics -- Section V. Sample Preparation and Analysis Techniques for Biological Fluids and Biomarker Discovery.- Section VI. Sample Preparation Methods in Plant Proteomics Section -- VII. Affinity Interaction and Biochemical Enrichment Techniques -- Section VIII. Methods for Quantitative proteomics -- Section IX. Sample Preparation in Functional Proteomics -- Section X. Characterization of Membrane Proteins.- Section XI. Methods for MALDI MS Enabled Biomedical Applications.- Section XII. Clinical Research and Applications.- Section XIII. Sample Preparation Techniques in Metabolomics and Drug Discovery -- Section XIV. Sample Preparation in Analysis or Exotic and Limited Availability Specimens -- Section XV. Sample Treatment in Biodefense, Forensics and Infectious Diseases -- Section XVI. Novel Approaches in Sample Preparation and LC-MS Analysis.
520 _aThe goal and philosophy of sample preparation in biological mass spectrometry is to reveal the actual multicomponent molecular structure of a biological specimen that can be assessed by mass spectrometry tools. Unfortunately, sample preparation is often looked at as a necessary but “boring” step which does not warrant any efforts beyond a paragraph or two in a “Methods” section; it is believed not to be conducive to innovation, and does not elicit the enthusiasm of funding agencies. Nevertheless, in recent years, the attention to sample preparation methods has risen considerably, both in Academia and in the life science industry. The aim of this book is to provide the researcher with important sample preparation strategies for a wide variety of analyte molecules, specimens, methods, and biological applications demanding mass spectrometric analysis as the detection end-point. In this volume we have compiled contributions from several laboratories that employ mass spectrometry for biological analysis. With the latest inventions and the introduction of highly sophisticated mass spectrometry equipment, sample preparation has become an extremely important bottleneck of biomedical analysis. This book is structured as a compilation of contributed chapters ranging from step-by-step protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.
650 0 _aChemistry.
650 0 _aMedicine.
650 0 _aAnalytical biochemistry.
650 0 _aMass spectrometry.
650 0 _aBiochemistry.
650 0 _aProteomics.
650 1 4 _aChemistry.
650 2 4 _aAnalytical Chemistry.
650 2 4 _aMass Spectrometry.
650 2 4 _aBiochemistry, general.
650 2 4 _aProteomics.
650 2 4 _aBiomedicine general.
650 2 4 _aBiophysics and Biological Physics.
700 1 _aLazarev, Alexander V.
_eeditor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9789400707580
856 4 0 _uhttp://dx.doi.org/10.1007/978-94-007-0828-0
912 _aZDB-2-CMS
999 _c109382
_d109382