000 03767nam a22005295i 4500
001 978-1-4419-9785-2
003 DE-He213
005 20140220083730.0
007 cr nn 008mamaa
008 110718s2011 xxu| s |||| 0|eng d
020 _a9781441997852
_9978-1-4419-9785-2
024 7 _a10.1007/978-1-4419-9785-2
_2doi
050 4 _aQH345
050 4 _aQD415-436
072 7 _aPSB
_2bicssc
072 7 _aSCI007000
_2bisacsh
082 0 4 _a572
_223
100 1 _aKatoch, Rajan.
_eauthor.
245 1 0 _aAnalytical Techniques in Biochemistry and Molecular Biology
_h[electronic resource] /
_cby Rajan Katoch.
264 1 _aNew York, NY :
_bSpringer New York :
_bImprint: Springer,
_c2011.
300 _aXVIII, 441p. 70 illus., 3 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aPreparation of solutions -- Expression of concentration -- Buffers and their preparation -- Techniques in biochemical evaluation -- Carbohydrate estimations -- Estimation of lipids -- Qualitative and quantitative estimations of amino acids and proteins -- Protein purification techniques -- Cell disruption and fractionation -- Enzymes in metabolism -- Isoenzyme analysis -- Chromatographic separations -- Methods for nutritional quality evaluation of food materials -- Nutritional evaluation of forages -- Techniques in molecular biology.
520 _aLong defined as a core subject in the life sciences, biochemistry now allows us to control living systems in ways undreamt of even a decade ago. Its relevance increases daily as plant biology and biotechnology move inexorably up the scientific agenda. This comprehensive volume explores the numerous experimental techniques that must be mastered by researchers in plant biology, biochemistry and biotechnology. As the interest in biochemical and molecular methods of investigating physiological processes rises, there's a concurrent development of new, faster, and more sensitive experimental procedures, allowing us to explore the inner workings of animal and plant organisms. <i>Analytical Techniques in Biochemistry and Molecular Biology</i> guides the reader through these methodologies, beginning with the preparation of solutions and the expression of their concentrates; continuing with the concept and preparation of buffers, a key element of working with enzymes; and discussing biochemical analysis, carbohydrates, and proteins and amino acids, as well as enzyme and isozyme analysis.  This volume also examines the full spectrum of protocols used in contemporary biochemical experimentation, including chromatographic separation procedures and the nutritional evaluation of foodstuffs. Lastly, the book covers cutting-edge techniques in biotechnology. Truly multidisciplinary, <i>Analytical Techniques in Biochemistry and Molecular Biology</i> is a valuable resource to researchers in biochemistry, biotechnology and their derivative fields.
650 0 _aLife sciences.
650 0 _aChemistry.
650 0 _aAnalytical biochemistry.
650 0 _aCarbohydrates.
650 0 _aBiochemistry.
650 0 _aEnzymes.
650 1 4 _aLife Sciences.
650 2 4 _aBiochemistry, general.
650 2 4 _aAnalytical Chemistry.
650 2 4 _aCarbohydrate Chemistry.
650 2 4 _aChemistry/Food Science, general.
650 2 4 _aProtein Science.
650 2 4 _aEnzymology.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9781441997845
856 4 0 _uhttp://dx.doi.org/10.1007/978-1-4419-9785-2
912 _aZDB-2-SBL
999 _c106123
_d106123