000			04064nam a22005535i 4500
001			978-1-4614-1390-5
003			DE-He213
005			20140220083242.0
007			cr nn 008mamaa
008			111024s2012 xxu| s |||| 0|eng d
020			_a9781461413905 _9978-1-4614-1390-5
024	7		_a10.1007/978-1-4614-1390-5 _2doi
050		4	_aTP248.65.F66
072		7	_aTDCT _2bicssc
072		7	_aTEC012000 _2bisacsh
082	0	4	_a641.3 _223
082	0	4	_a664 _223
100	1		_aŽel, Jana. _eauthor.
245	1	0	_aHow to Reliably Test for GMOs _h[electronic resource] / _cby Jana Žel, Mojca Milavec, Dany Morisset, Damien Plan, Guy Van den Eede, Kristina Gruden.
264		1	_aBoston, MA : _bSpringer US : _bImprint: Springer, _c2012.
300			_aX, 100p. 21 illus., 19 illus. in color. _bonline resource.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_atext file _bPDF _2rda
490	1		_aSpringerBriefs in Food, Health, and Nutrition
505	0		_aIntroduction -- Legislation on GMO Labelling and GMO Detection -- Organization of the Laboratory and Quality Management System -- Methods -- Verification of Methods -- Homogenization and DNA Extraction -- Real-Time PCR -- Measurement Uncertainty -- New Challenges -- References -- Index.
520			_aThe detection of genetically modified organisms (GMOs) is becoming very complex, with new GMOs, approved and unapproved, constantly entering world markets. Traceability and labelling of GMOs is defined in regulations worldwide, demanding accurate and reliable testing to support the requirements of legislation. This Brief provides the current state-of-the-art on all key topics involved in GMO testing and is a source of detailed practical information for laboratories. Special focus is given to qualitative and quantitative real-time PCR analysis relevant to all areas where detection and identification rely on nucleic acid-based methods. The following topics, important for testing laboratories, are also discussed: organization of the laboratory, focusing on aspects of the quality system and methods for testing, validation and verification of methods, and measurement uncertainty. The Brief also discusses the new challenges of GMOs and novel modified organisms, using new technologies, and the possible solutions for GMO detection, including bioinformatics tools. Finally, legislation on GMOs and sources of information on GMOs are provided, which are relevant not only to testing laboratories, but to anyone interested in GMOs. The authors of this Brief have many years of experience in GMO testing, development of real-time PCR methods, implementation of quality system requirements, validations and verification of methods, and measurement uncertainty. The National Institute of Biology is a highly qualified research laboratory and a National Reference Laboratory, which also performs routine analyses of food, feed and seed. The Institute for Health and Consumer Protection of the European Union Joint Research Centre has extensive knowledge and experience of GMO detection. It hosts the European Union Reference Laboratory for GM Food and Feed in addition to chairing the European Network of GMO Laboratories.
650		0	_aChemistry.
650		0	_aGenetic engineering.
650		0	_aFood science.
650		0	_aMicrobiology.
650	1	4	_aChemistry.
650	2	4	_aFood Science.
650	2	4	_aGenetic Engineering.
650	2	4	_aMicrobiology.
700	1		_aMilavec, Mojca. _eauthor.
700	1		_aMorisset, Dany. _eauthor.
700	1		_aPlan, Damien. _eauthor.
700	1		_aVan den Eede, Guy. _eauthor.
700	1		_aGruden, Kristina. _eauthor.
710	2		_aSpringerLink (Online service)
773	0		_tSpringer eBooks
776	0	8	_iPrinted edition: _z9781461413899
830		0	_aSpringerBriefs in Food, Health, and Nutrition
856	4	0	_uhttp://dx.doi.org/10.1007/978-1-4614-1390-5
912			_aZDB-2-CMS
999			_c101057 _d101057