Phosphorescent Oxygen-Sensitive Probes [electronic resource] / by Dmitri B. Papkovsky, Alexander V. Zhdanov, Andreas Fercher, Ruslan I. Dmitriev, James Hynes.
By: Papkovsky, Dmitri B [author.].
Contributor(s): Zhdanov, Alexander V [author.] | Fercher, Andreas [author.] | Dmitriev, Ruslan I [author.] | Hynes, James [author.] | SpringerLink (Online service).
Material type: BookSeries: SpringerBriefs in Biochemistry and Molecular Biology: Publisher: Basel : Springer Basel : Imprint: Springer, 2012Description: VIII, 101 p. 39 illus., 17 illus. in color. online resource.Content type: text Media type: computer Carrier type: online resourceISBN: 9783034805254.Subject(s): Medicine | Biochemical engineering | Cytology | Microbiology | Cytology -- Research_xMethodology | Biomedical engineering | Biomedicine | Molecular Medicine | Biological Techniques | Biochemical Engineering | Cell Biology | Microbiology | Biomedical EngineeringDDC classification: 611.01816 Online resources: Click here to access onlineO2-sensitive probes based on phosphorescent metalloporphyrins -- O2 analysis on a fluorescence spectrometer or plate reader -- O2 Imaging in biological specimens.
Biological O2 sensing probes and measurement techniques were first introduced in the late 80s. In the last 3-5 years they have undergone major development that have made them available and affordable for a broad range of applications in various disciplines of the life and biomedical sciences. These new chemistries and technologies, which are significantly different from the majority of other fluorescence-based probes and detection techniques, have already demonstrated their high utility. This book will provide a systematic overview of the existing and emerging O2 sensing technologies in their different modifications, a practical guide to their rational selection and use, and examples of biological applications/case studies, including details on how to set up and conduct such experiments, troubleshoot and interpret the data.
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